Enzymes in a golden cage.

ABSTRACT: We describe a general method for the entrapment of enzymes within bulk metallic gold. This is a new approach for the immobilization of enzymes on metals, which is commonly carried out by 2D adsorption or covalent biding, that is, the enzyme is in contact with the metal at a specific contact zone of the enzyme, while most of the rest of it remains exposed to the environment. The 3D metallic encaging of the enzymes is quite different: the enzyme is in contact with the metallic cage walls all around it and is well protected inside. The porous nature of the metallic matrix enables substrate molecules to diffuse inside, reach the active site, and let product molecules diffuse out. The generality of the approach was proven by the successful entrapment of five enzymes representing different class

SUBMITTER: Baruch-Shpigler Y 

PROVIDER: S-EPMC8152684 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature