Templates direct the sequence-specific anchoring of the C-terminus of peptido RNAs.

ABSTRACT: When amino acids and ribonucleotides react in aqueous condensation buffer, they form peptido RNA with a phosphoramidate bond between the N-terminus of the peptide and the 5'-terminal phosphate of a ribonucleotide. If peptido RNA was the product of spontaneous reactions of amino acids and nucleotides, there must have been a transition to peptidyl tRNAs, where the C-terminus of the peptide is ester-linked to the 2',3'-terminus of an oligonucleotide. Here we report how short peptido RNAs react with the 3'-terminus of oligodeoxynucleotides, templated by RNA strands. In our model system, the rate and yield of the anchoring of the C-terminus of the dipeptido dinucleotides to an amino group was found to depend on the sequence of the peptide, the 5'-terminal nucleotide of the

SUBMITTER: Jash B 

PROVIDER: S-EPMC8152794 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature