Using NMR Spectroscopy To Measure Protein Binding Capacity on Gold Nanoparticles.

ABSTRACT: A simple one-dimensional ¹H NMR experiment that quantifies protein bound to gold nanoparticles has been developed for upper-division biochemistry and physical chemistry students. This laboratory experiment teaches the basics of NMR techniques, which is a highly effective tool in protein studies and supports students to understand the concepts of NMR spectroscopy and nanoparticle-protein interactions. Understanding the interactions of gold nanoparticles (AuNPs) with biological macromolecules is becoming increasingly important as interest in the clinical use of nanoparticles has been on the rise. Applications in drug delivery, biosensing, diagnostics, and enhanced imaging are all tangible possibilities with a better understanding of AuNP-protein interactions. The ability to use Au

SUBMITTER: Perera YR 

PROVIDER: S-EPMC8152810 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature