Genome-Wide Analysis Identifies an Essential Human TBX3 Pacemaker Enhancer.

ABSTRACT:

Rationale

The development and function of the pacemaker cardiomyocytes of the sinoatrial node (SAN), the leading pacemaker of the heart, are tightly controlled by a conserved network of transcription factors, including TBX3 (T-box transcription factor 3), ISL1 (ISL LIM homeobox 1), and SHOX2 (short stature homeobox 2). Yet, the regulatory DNA elements (REs) controlling target gene expression in the SAN pacemaker cells have remained undefined.

Objective

Identification of the regulatory landscape of human SAN-like pacemaker cells and functional assessment of SAN-specific REs potentially involved in pacemaker cell gene regulation.

Methods and results

We performed Assay for Transposase-Accessible Chromatin using sequencing on human pluripotent stem cell-derived SAN-like p

SUBMITTER: van Eif VWW 

PROVIDER: S-EPMC8153223 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature