Identification and Validation of Reference Genes for Gene Expression Analysis in Schima superba.

ABSTRACT: Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene expression studies, but its accuracy depends on the expression stability of reference genes. Schima superba is a fast-growing timber species with strong resistance. However, thus far, reliable reference gene identifications have not been reported in S. superba. In this study, 19 candidate reference genes were selected and evaluated for their expression stability in different tissues of S. superba. Three software programs (geNorm, NormFinder, and BestKeeper) were used to evaluate the reference gene transcript stabilities, and comprehensive stability ranking was generated by the geometric mean method. Our results show that SsuACT was the most stable reference gene and that

SUBMITTER: Yang Z 

PROVIDER: S-EPMC8153319 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature