Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.

ABSTRACT: Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmental mercury. Both single-signal and double-signal output mercury biosensors were assembled using a natural mer operon as a template. Selectivity and sensitivity of whole-cell biosensors based on artificial mer operons were determined. Three whole-cell biosensors were highly stable at very high concentrations of mercuric chloride, and could detect bioavailable Hg(II) in the concentration range of 6.25-200 μM HgCl2. A novel Hg(II) bioadsorption coupled with biosensing artificial mer operon was ass

SUBMITTER: Zhang NX 

PROVIDER: S-EPMC8153442 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature