Optimum DNA Extraction Methods for Edible Bird's Nest Identification Using Simple Additive Weighting Technique.

ABSTRACT: A simple additive weighting (SAW) technique was used to determine and compare the overall performance of five DNA extraction methods from conventional (SDS method) to commercial kits (Qiagen, Wizard, and NucleoSpin) for identifying origins of edible bird's nest (EBN) using end-point polymerase chain reaction (PCR). A hybrid method (SDS/Qiagen) which has been developed by combining the conventional SDS method with commercialised Qiagen was determined as the most suitable in terms of speed and cost-effectiveness. The determination of optimum extraction method was by the performances on efficiency and feasibility, extracted DNA concentration, purity, PCR amplifiability, handling time and safety of reagents used. The hybrid SDS/Qiagen method is less costly compared to the commercial kits and o

SUBMITTER: Quek MC 

PROVIDER: S-EPMC8153580 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature