Substrate Specificity and Biochemical Characteristics of an Engineered Mammalian Chondroitinase ABC.

ABSTRACT: Chondroitin sulfate proteoglycans inhibit regeneration, neuroprotection, and plasticity following spinal cord injury. The development of a second-generation chondroitinase ABC enzyme, capable of being secreted from mammalian cells (mChABC), has facilitated the functional recovery of animals following severe spinal trauma. The genetically modified enzyme has been shown to efficiently break down the inhibitory extracellular matrix surrounding cells at the site of injury, while facilitating cellular integration and axonal growth. However, the activity profile of the enzyme in relation to the original bacterial chondroitinase (bChABC) has not been determined. Here, we characterize the activity profile of mChABC and compare it to bChABC, both enzymes having been maintained under physiologically

SUBMITTER: Warren PM 

PROVIDER: S-EPMC8153898 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature