Three-dimensional reconstruction of systematic histological sections: application to observations on palatal shelf elevation.

ABSTRACT: Normal mammalian secondary palate development undergoes a series of processes, including palatal shelf (PS) growth, elevation, adhesion and fusion, and palatal bone formation. It has been estimated that more than 90% of isolated cleft palate is caused by defects associated with the elevation process. However, because of the rapidly completed elevation process, the entire process of elevation will never be easy to clarify. In this article, we present a novel method for three-dimensional (3D) reconstruction of thick tissue blocks from two-dimensional (2D) histological sections. We established multiplanar sections of the palate and tongue in coronal and sagittal directions, and further performed 3D reconstruction to observe the morphological interaction and connection between the two componen

SUBMITTER: Liu W 

PROVIDER: S-EPMC8154959 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature