Whole brain functional recordings at cellular resolution in zebrafish larvae with 3D scanning multiphoton microscopy.

ABSTRACT: Optical recordings of neuronal activity at cellular resolution represent an invaluable tool to investigate brain mechanisms. Zebrafish larvae is one of the few model organisms where, using fluorescence-based reporters of the cell activity, it is possible to optically reconstruct the neuronal dynamics across the whole brain. Typically, leveraging the reduced light scattering, methods like lightsheet, structured illumination, and light-field microscopy use spatially extended excitation profiles to detect in parallel activity signals from multiple cells. Here, we present an alternative design for whole brain imaging based on sequential 3D point-scanning excitation. Our approach relies on a multiphoton microscope integrating an electrically tunable lens. We first apply our approach, adopting t

SUBMITTER: Bruzzone M 

PROVIDER: S-EPMC8154985 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature