Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans.

ABSTRACT: Genetic mapping is used in forward genetics to narrow the list of candidate mutations and genes corresponding to the mutant phenotype of interest. Even with modern advances in biology such as efficient identification of candidate mutations by whole-genome sequencing, mapping remains critical in pinpointing the responsible mutation. Here we describe a simple, fast, and affordable mapping toolkit that is particularly suitable for mapping in Caenorhabditis elegans. This mapping method uses insertion-deletion polymorphisms or indels that could be easily detected instead of single nucleotide polymorphisms in commonly used Hawaiian CB4856 mapping strain. The materials and methods were optimized so that mapping could be performed using tiny amount of genetic material without growing many large po

SUBMITTER: Hwang HY 

PROVIDER: S-EPMC8155061 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature