Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.

ABSTRACT: Compact cardiomyocytes that make up the ventricular wall of the adult heart represent an important therapeutic target population for modeling and treating cardiovascular diseases. Here, we established a differentiation strategy that promotes the specification, proliferation and maturation of compact ventricular cardiomyocytes from human pluripotent stem cells (hPSCs). The cardiomyocytes generated under these conditions display the ability to use fatty acids as an energy source, a high mitochondrial mass, well-defined sarcomere structures and enhanced contraction force. These ventricular cells undergo metabolic changes indicative of those associated with heart failure when challenged in vitro with pathological stimuli and were found to generate grafts consisting of more mature cells than th

SUBMITTER: Funakoshi S 

PROVIDER: S-EPMC8155185 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature