Serum and Plasma Immunoglobulin G Fc N-Glycosylation Is Stable during Storage.

ABSTRACT: Immunoglobulin G (IgG) glycosylation is studied in biological samples to develop clinical markers for precision medicine, for example, in autoimmune diseases and oncology. Inappropriate storage of proteins, lipids, or metabolites can lead to degradation or modification of biomolecular features, which can have a strong negative impact on accuracy and precision of clinical omics studies. Regarding the preservation of IgG glycosylation, the range of appropriate storage conditions and time frame is understudied. Therefore, we investigated the effect of storage on IgG Fc N-glycosylation in the commonly analyzed biofluids, serum and plasma. Short-term storage and accelerated storage stability were tested by incubating samples from three healthy donors under stress conditions of up to 50 °C for 2

SUBMITTER: Amez Martin M 

PROVIDER: S-EPMC8155565 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature