Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration.

ABSTRACT: Treatment of inflammatory bowel disease (IBD) is challenging, with a series of available drugs each helping only a fraction of patients. Patients may face time-consuming drug trials while the disease is active, thus there is an unmet need for biomarkers and assays to predict drug effect. It is well known that the intestinal epithelium is an important factor in disease pathogenesis, exhibiting physical, biochemical and immunologic driven barrier dysfunctions. One promising test system to study effects of existing or emerging IBD treatments targeting intestinal epithelial cells (IECs) is intestinal organoids ("mini-guts"). However, the fact that healthy intestinal epithelium is in a physiologically hypoxic state has largely been neglected, and studies with intestinal organoids are mainly per

SUBMITTER: Skovdahl HK 

PROVIDER: S-EPMC8156408 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature