A pan-genome method to determine core regions of the Bacillus subtilis and Escherichia coli genomes.

ABSTRACT: Background: Synthetic engineering of bacteria to produce industrial products is a burgeoning field of research and application. In order to optimize genome design, designers need to understand which genes are essential, which are optimal for growth, and locations in the genome that will be tolerated by the organism when inserting engineered cassettes. Methods: We present a pan-genome based method for the identification of core regions in a genome that are strongly conserved at the species level. Results: We show that the core regions determined by our method contain all or almost all essential genes. This demonstrates the accuracy of our method as essential genes should be core genes. We show that we outperform previous methods by this measure. We also explain why ther

SUBMITTER: Sutton G 

PROVIDER: S-EPMC8156514 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature