Current Practice in Bicistronic IRES Reporter Use: A Systematic Review.

ABSTRACT: Bicistronic reporter assays have been instrumental for transgene expression, understanding of internal ribosomal entry site (IRES) translation, and identification of novel cap-independent translational elements (CITE). We observed a large methodological variability in the use of bicistronic reporter assays and data presentation or normalization procedures. Therefore, we systematically searched the literature for bicistronic IRES reporter studies and analyzed methodological details, data visualization, and normalization procedures. Two hundred fifty-seven publications were identified using our search strategy (published 1994-2020). Experimental studies on eukaryotic adherent cell systems and the cell-free translation assay were included for further analysis. We evaluated the following metho

SUBMITTER: van den Akker GGH 

PROVIDER: S-EPMC8156625 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature