Impact of Extraction Method on the Detection of Quality Biomarkers in Normal vs. DFD Meat.

ABSTRACT: The objective of this work was to demonstrate how the extraction method affects the reliability of biomarker detection and how this detection depends on the biomarker location within the cell compartment. Different extraction methods were used to study the sarcoplasmic and myofibrillar fractions of the Longissimus thoracis et lumborum muscle of young bulls of the Asturiana de los Valles breed in two quality grades, standard (Control) or dark, firm, and dry (DFD) meat. Protein extractability and the expression of some of the main meat quality biomarkers-oxidative status (lipoperoxidation (LPO) and catalase activity (CAT)), proteome (SDS-PAGE electrophoretic pattern), and cell stress protein (Hsp70)-were analyzed. In the sarcoplasmic fraction, buffers containing Triton X-100 showed si

SUBMITTER: Gonzalez-Blanco L 

PROVIDER: S-EPMC8156798 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature