NBAT1/CASC15-003/USP36 control MYCN expression and its downstream pathway genes in neuroblastoma.

ABSTRACT:

Background

MYCN has been an attractive therapeutic target in neuroblastoma considering the widespread amplification of the MYCN locus in neuroblastoma, and its established role in neuroblastoma development and progression. Thus, understanding neuroblastoma-specific control of MYCN expression at the transcriptional and post-transcriptional level would lead to identification of novel MYCN-dependent oncogenic pathways and potential therapeutic strategies.

Methods

By performing loss- and gain-of-function experiments of the neuroblastoma hotspot locus 6p22.3 derived lncRNAs CASC15-003 and NBAT1, together with coimmunoprecipitation and immunoblotting of MYCN, we have shown that both lncRNAs post-translationally control the expression of MYCN through reg

SUBMITTER: Juvvuna PK 

PROVIDER: S-EPMC8156975 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature