The accumulation of exosome-associated microRNA-1246 and microRNA-150-3p in human red blood cell suspensions.

ABSTRACT:

Background

Transfusion-related immunomodulation (TRIM) can be caused by exosomes, in which case, microRNAs (miRNAs) are one critical factor impacting exosome behavior. This study aims to investigate and analyze the expression profiles of exosomal miRNA in red blood cell (RBC) suspensions during storage and to identify potential TRIM-related miRNAs as well as their potential functions.

Methods

A total of 25 packs of RBC suspensions were randomly collected. Exosome were extracted by ultracentrifugation and then identified and characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and western blot (WB). Exosomal miRNA profiles were acquired using gene chips in five packs on week 1 and week 5. The expression data were compared from the two

SUBMITTER: Kong Y 

PROVIDER: S-EPMC8157439 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature