Distal Enhancer Potentiates Activin- and GnRH-Induced Transcription of FSHB.

ABSTRACT: FSH is critical for fertility. Transcription of FSHB, the gene encoding the beta subunit, is rate-limiting in FSH production and is regulated by both GnRH and activin. Activin signals through SMAD transcription factors. Although the mechanisms and importance of activin signaling in mouse Fshb transcription are well-established, activin regulation of human FSHB is less well understood. We previously reported a novel enhancer of FSHB that contains a fertility-associated single nucleotide polymorphism (rs10031006) and requires a region resembling a full (8 base-pair) SMAD binding element (SBE). Here, we investigated the role of the putative SBE within the enhancer in activin and GnRH regulation of FSHB. In mouse gonadotrope-derived LβT2 cells, the upstream enhancer potentiated activin inducti

SUBMITTER: Bohaczuk SC 

PROVIDER: S-EPMC8157479 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature