Inhibition of herpes simplex virus-1 infection by MBZM-N-IBT: in silico and in vitro studies.

ABSTRACT:

Introduction

The emergence of drug resistance and cross-resistance to existing drugs has warranted the development of new antivirals for Herpes simplex viruses (HSV). Hence, we have designed this study to evaluate the anti-viral activity of 1-[(2-methyl benzimidazole-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea (MBZM-N-IBT), against HSV-1.

Method

Molecular docking was performed to assess the affinity of MBZM-N-IBT for HSV-1 targets. This was validated by plaque assay, estimation of RNA and protein levels as well as time of addition experiments in vitro.

Result

Molecular docking analysis suggested the inhibitory capacity of MBZM-N-IBT against HSV-1. This was supported by the abrogation of the HSV-1 infectious viral particle formation with the IC₅₀

SUBMITTER: Kumar A 

PROVIDER: S-EPMC8157732 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature