Ovomucoid epitope-specific repertoire of IgE, IgG₄ , IgG₁ , IgA₁ , and IgD antibodies in egg-allergic children.

ABSTRACT:

Background

Egg-white ovomucoid, that is, Gal d 1, is associated with IgE-mediated allergic reactions in most egg-allergic children. Epitope-specific IgE levels have been correlated with the severity of egg allergy, while emerging evidence suggests that other antibody isotypes (IgG₁ , IgG₄ , IgA, and IgD) may have a protective function; yet, their epitope-specific repertoires and associations with atopic comorbidities have not been studied.

Methods

Bead-based epitope assay (BBEA) was used to quantitate the levels of epitope-specific (es)IgA, esIgE, esIgD, esIgG₁ , and esIgG₄ antibodies directed at 58 (15-mer) overlapping peptides, covering the entire sequence of ovomucoid, in plasma of 38 egg-allergic and 6 atopic children. Intracla

SUBMITTER: Suprun M 

PROVIDER: S-EPMC8158022 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature