Protein Extraction From FFPE Kidney Tissue Samples: A Review of the Literature and Characterization of Techniques.

ABSTRACT: Most tissue biopsies from patients in hospital environments are formalin-fixed and paraffin-embedded (FFPE) for long-term storage. This fixation process produces a modification in the proteins called "crosslinks", which improves protein stability necessary for their conservation. Currently, these samples are mainly used in clinical practice for performing immunohistochemical analysis, since these modifications do not suppose a drawback for this technique; however, crosslinks difficult the protein extraction process. Accordingly, these modifications make the development of a good protein extraction protocol necessary. Due to the specific characteristics of each tissue, the same extraction buffers or deparaffinization protocols are not equally effective in all cases. Therefore, it is necessa

SUBMITTER: Garcia-Vence M 

PROVIDER: S-EPMC8158658 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature