Multivalent Affinity Profiling: Direct Visualization of the Superselective Binding of Influenza Viruses.

ABSTRACT: The influenza A virus (IAV) interacts with the glycocalyx of host cells through its surface proteins hemagglutinin (HA) and neuraminidase (NA). Quantitative biophysical measurements of these interactions may help to understand these interactions at the molecular level with the long-term aim to predict influenza infectivity and answer other biological questions. We developed a method, called multivalent affinity profiling (MAP), to measure virus binding profiles on receptor density gradients to determine the threshold receptor density, which is a quantitative measure of virus avidity toward a receptor. Here, we show that imaging of IAVs on receptor density gradients allows the direct visualization and efficient assessment of their superselective binding. We show how the multivalent binding

SUBMITTER: Overeem NJ 

PROVIDER: S-EPMC8158855 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature