PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples.

ABSTRACT: A fast PCR-assisted impedimetric biosensor was developed for the selective detection of the clbN gene from the polyketide synthase (pks) genomic island in real Escherichia coli samples. This genomic island is responsible for the production of colibactin, a harmful genotoxin that has been associated with colorectal cancer. The experimental protocol consisted of immobilizing the designated forward primer onto an Au electrode surface to create the sensing probe, followed by PCR temperature cycling in blank, positive, and negative DNA controls. Target DNA identification was possible by monitoring changes in the system's charge transfer resistance values (R_ct) before and after PCR treatment through electrochemical impedance spectroscopy (EIS) analysis. Custom-made, flexible gold elec

SUBMITTER: Solis-Marcano NE 

PROVIDER: S-EPMC8159250 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature