SMolESY: an efficient and quantitative alternative to on-instrument macromolecular ¹H-NMR signal suppression.

ABSTRACT: One-dimensional (1D) proton-nuclear magnetic resonance (¹H-NMR) spectroscopy is an established technique for measuring small molecules in a wide variety of complex biological sample types. It is demonstrably reproducible, easily automatable and consequently ideal for routine and large-scale application. However, samples containing proteins, lipids, polysaccharides and other macromolecules produce broad signals which overlap and convolute those from small molecules. NMR experiment types designed to suppress macromolecular signals during acquisition may be additionally performed, however these approaches add to the overall sample analysis time and cost, especially for large cohort studies, and fail to produce reliably quantitative data. Here, we propose an alternative way of compu

SUBMITTER: Takis PG 

PROVIDER: S-EPMC8159292 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature