Mixing and matching genes of marine and terrestrial origin in the biosynthesis of the mupirocin antibiotics.

ABSTRACT: With growing understanding of the underlying pathways of polyketide biosynthesis, along with the continual expansion of the synthetic biology toolkit, it is becoming possible to rationally engineer and fine-tune the polyketide biosynthetic machinery for production of new compounds with improved properties such as stability and/or bioactivity. However, engineering the pathway to the thiomarinol antibiotics has proved challenging. Here we report that genes from a marine Pseudoalternomonas sp. producing thiomarinol can be expressed in functional form in the biosynthesis of the clinically important antibiotic mupirocin from the soil bacterium Pseudomonas fluorescens. It is revealed that both pathways employ the same unusual mechanism of tetrahydropyran (THP) ring formation and th

SUBMITTER: Wang L 

PROVIDER: S-EPMC8159325 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature