Introducing charge tag via click reaction in living cells for single cell mass spectrometry.

ABSTRACT: For single living cell mass spectrometry measurement, sensitivity is of great significance due to the extremely complicated chemical components of the cytoplasm. Higher sensitivity is always highly desired, especially for chemicals with low concentrations or poor mass spectrometry responses. Here, a quaternary ammonium salt group-based charge tag was designed to enhance the analytical performance for cysteine within single cells using induced nanoelectrospray mass spectrometry. While the charge tag was coupled to the analyte via biocompatible click reaction, viability of the living cells was maintained during in situ derivatization and following analysis. Enhanced sensitivity under physiological conditions for cysteine, at pH 7.4 and with highly concentrated salts, was achiev

SUBMITTER: Zhuang M 

PROVIDER: S-EPMC8159379 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature