Investigation and improvement of catalytic activity of G-quadruplex/hemin DNAzymes using designed terminal G-tetrads with deoxyadenosine caps.

ABSTRACT: It is generally acknowledged that G-quadruplexes (G4s) acquire peroxidase activity upon interaction with hemin. Hemin has been demonstrated to bind selectively to the 3'-terminal G-tetrad of parallel G4s via end-stacking; however, the relationships between different terminal G-tetrads and the catalytic functions of G4/hemin DNAzymes are not fully understood. Herein, the oligonucleotide d(AGGGGA) and its three analogues, d(AG^BrG^BrGGA), d(AG^BrGGG^BrA) and d(AG^BrGG^BrGA) (G^Br indicates 8-bromo-2'-deoxyguanosine), were designed. These oligonucleotides form three parallel G4s and one antiparallel G4 without loop regions. The scaffolds had terminal G-tetrads that were either anti-deoxyguanosines (anti

SUBMITTER: Cao Y 

PROVIDER: S-EPMC8159390 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature