A fluorescent molecular imaging probe with selectivity for soluble tau aggregated protein.

ABSTRACT: Soluble forms of aggregated tau misfolded protein, generally termed oligomers, are considered to be the most toxic species of the different assembly states that are the pathological components of neurodegenerative disorders. Therefore, a critical biomedical need exists for imaging probes that can identify and quantify them. We have designed and synthesized a novel fluorescent probe, pTP-TFE for which binding and selectivity profiles towards aggregated tau and Aβ proteins were assessed. Our results have shown pTP-TFE to be selective for early forms of soluble tau aggregates, with high affinity of dissociation constants (K _d) = 66 nM, and tenfold selectivity over mature tau fibrils. Furthermore, we found that pTP-TFE is selective for tau over Aβ aggrega

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8159431 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature