Intestinal Virome in Patients With Alcoholic Hepatitis.

ABSTRACT:

Background and aims

Alcoholic hepatitis (AH) is a severe manifestation of alcohol-associated liver disease (ALD) with high mortality. Although gut bacteria and fungi modulate disease severity, little is known about the effects of the viral microbiome (virome) in patients with ALD.

Approach and results

We extracted virus-like particles from 89 patients with AH who were enrolled in a multicenter observational study, 36 with alcohol use disorder (AUD), and 17 persons without AUD (controls). Virus-like particles from fecal samples were fractionated using differential filtration techniques, and metagenomic sequencing was performed to characterize intestinal viromes. We observed an increased viral diversity in fecal samples from patients with ALD, with the most significant changes

SUBMITTER: Jiang L 

PROVIDER: S-EPMC8159727 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature