Nuclear m⁶A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.

ABSTRACT: N⁶-methyladenosine (m⁶A) on chromosome-associated regulatory RNAs (carRNAs), including repeat RNAs, plays important roles in tuning the chromatin state and transcription, but the intrinsic mechanism remains unclear. Here, we report that YTHDC1 plays indispensable roles in the self-renewal and differentiation potency of mouse embryonic stem cells (ESCs), which highly depends on the m⁶A-binding ability. Ythdc1 is required for sufficient rRNA synthesis and repression of the 2-cell (2C) transcriptional program in ESCs, which recapitulates the transcriptome regulation by the LINE1 scaffold. Detailed analyses revealed that YTHDC1 recognizes m⁶A on LINE1 RNAs in the nucleus and regulates the formation of the LINE1-NCL partnership and the chromatin recru

SUBMITTER: Chen C 

PROVIDER: S-EPMC8160034 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature