Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.

ABSTRACT: Reverse transcription quantitative real-time PCR is the most commonly used method to detect gene expression levels. In experiments, it is often necessary to correct and standardize the expression level of target genes with reference genes. Therefore, it is very important to select stable reference genes to obtain accurate quantitative results. Although application examples of reference genes in mammals have been reported, no studies have investigated the use of reference genes in studying the growth and development of adipose tissue and the proliferation and differentiation of preadipocytes in chickens. In this study, GeNorm, a reference gene stability statistical algorithm, was used to analyze the expression stability of 14 candidate reference genes in the abdominal adipose tissue of broi

SUBMITTER: Na W 

PROVIDER: S-EPMC8160385 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature