Establishing a 3D In Vitro Hepatic Model Mimicking Physiologically Relevant to In Vivo State.

ABSTRACT: Three-dimensional (3D) bioprinting is a promising technology to establish a 3D in vitro hepatic model that holds great potential in toxicological evaluation. However, in current hepatic models, the central area suffers from hypoxic conditions, resulting in slow and weak metabolism of drugs and toxins. It remains challenging to predict accurate drug effects in current bioprinted hepatic models. Here, we constructed a hexagonal bioprinted hepatic construct and incorporated a spinning condition with continuous media stimuli. Under spinning conditions, HepG2 cells in the bioprinted hepatic construct exhibited enhanced proliferation capacity and functionality compared to those under static conditions. Additionally, the number of spheroids that play a role in boosting drug-induced signals and re

SUBMITTER: Kang HK 

PROVIDER: S-EPMC8161177 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature