Recombinant Antibody Production Using a Dual-Promoter Single Plasmid System.

ABSTRACT: Monoclonal antibodies (mAbs) have demonstrated tremendous effects on the treatment of various disease indications and remain the fastest growing class of therapeutics. Production of recombinant antibodies is performed using mammalian expression systems to facilitate native antibody folding and post-translational modifications. Generally, mAb expression systems utilize co-transfection of heavy chain (hc) and light chain (lc) genes encoded on separate plasmids. In this study, we examine the production of two FDA-approved antibodies using a bidirectional (BiDi) vector encoding both hc and lc with mirrored promoter and enhancer elements on a single plasmid, by analysing the individual hc and lc mRNA expression levels and subsequent quantification of fu

SUBMITTER: Carrara SC 

PROVIDER: S-EPMC8161450 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature