Room-temperature crystallography using a microfluidic protein crystal array device and its application to protein-ligand complex structure analysis.

ABSTRACT: Room-temperature (RT) protein crystallography provides significant information to elucidate protein function under physiological conditions. In particular, contrary to typical binding assays, X-ray crystal structure analysis of a protein-ligand complex can determine the three-dimensional (3D) configuration of its binding site. This allows the development of effective drugs by structure-based and fragment-based (FBDD) drug design. However, RT crystallography and RT crystallography-based protein-ligand complex analyses require the preparation and measurement of numerous crystals to avoid the X-ray radiation damage. Thus, for the application of RT crystallography to protein-ligand complex analysis, the simultaneous preparation of protein-ligand complex crystals and sequential X-ray diffractio

SUBMITTER: Maeki M 

PROVIDER: S-EPMC8162031 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature