Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo.

ABSTRACT: The α S 2 -casein ( α S 2 -CN) is a member of the casein family associated with milk traits in ruminants, but so far the buffalo CSN1S2 gene has not been well understood. In this work, the polymorphisms of CSN1S2 in river and swamp buffalo were detected using direct sequencing of polymerase chain reaction (PCR) products. As a result, 13 single nucleotide polymorphisms (SNPs) were identified in the coding sequence (CDS) of CSN1S2 in two types of buffalo, of which eight SNPs were non-synonymous. The amino acid changes caused by c.580T > C and c.642C > G may affect the function of buffalo α S 2 -CN. A total of 11 CSN1S2 CDS haplotypes were defined, and accordingly 11 variants of buffalo α S 2 -CN were inferred and named. The CSN1S2 CDSs of bo

SUBMITTER: Fan X 

PROVIDER: S-EPMC8162411 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature