A serological aptamer-assisted proximity ligation assay for COVID-19 diagnosis and seeking neutralizing aptamers.

ABSTRACT: Rapid and accurate diagnosis of COVID-19 plays an essential role in the current epidemic prevention and control. Despite the promise of nucleic acid and antibody tests, there is still a great challenge to reduce the misdiagnosis, especially for asymptomatic individuals. Here we report a generalizable method for highly specific and ultrasensitive detection of serum COVID-19-associated antigens based on an aptamer-assisted proximity ligation assay. The sensor is based on binding two aptamer probes to the same protein target that brings the ligation DNA region into close proximity, thereby initiating ligation-dependent qPCR amplification. Using this system, serum nucleocapsid protein has been detected quantitatively by converting protein recognition into a detectable qPCR signal using a simpl

SUBMITTER: Liu R 

PROVIDER: S-EPMC8162504 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature