Protamine Characterization by Top-Down Proteomics: Boosting Proteoform Identification with DBSCAN.

ABSTRACT: Protamines replace histones as the main nuclear protein in the sperm cells of many species and play a crucial role in compacting the paternal genome. Human spermatozoa contain protamine 1 (P1) and the family of protamine 2 (P2) proteins. Alterations in protamine PTMs or the P1/P2 ratio may be associated with male infertility. Top-down proteomics enables large-scale analysis of intact proteoforms derived from alternative splicing, missense or nonsense genetic variants or PTMs. In contrast to current gold standard techniques, top-down proteomics permits a more in-depth analysis of protamine PTMs and proteoforms, thereby opening up new perspectives to unravel their impact on male fertility. We report on the analysis of two normozoospermic semen samples by top-down proteomics. We discuss the d

SUBMITTER: Arauz-Garofalo G 

PROVIDER: S-EPMC8162566 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature