Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next-generation sequencing.

ABSTRACT:

Background

Urine from clinically healthy dogs is not sterile. Characterizing microbial diversity and abundance within this population of dogs is important to define normal reference ranges for healthy urine.

Objectives

To establish composition and relative representation of bacterial and fungal microbiomes in urine of clinically healthy dogs.

Animals

Fifty clinically healthy dogs.

Methods

Analytic study. Urine sampling via cystocentesis. Comprehensive evaluation of urine including standard urinalysis, culture and sensitivity, next-generation sequencing (NGS), and bioinformatics to define bacterial and fungal microbiome.

Results

Culture did not yield positive results in any samples. Next-generation sequencing of urine established low presence of bacteria,

SUBMITTER: Melgarejo T 

PROVIDER: S-EPMC8162589 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature