Validation of Histoplasmosis Enzyme Immunoassay to Evaluate Suspicious Lung Nodules.

ABSTRACT:

Background

Granulomas caused by infectious lung diseases can present as indeterminate pulmonary nodules (IPN). This study aims to validate an enzyme immunoassay (EIA) for Histoplasma immunoglobulin G (IgG) and immunoglobulin M (IgM) for diagnosing benign IPN in areas with endemic histoplasmosis.

Methods

Prospectively collected serum samples from patients at Vanderbilt University Medical Center (VUMC [n = 204]), University of Pittsburgh Medical Center (n = 71), and University of Cincinnati (n = 51) with IPN measuring 6 to 30 mm were analyzed for Histoplasma IgG and IgM with EIA. Diagnostic test characteristics were compared with results from the VUMC pilot cohort (n = 127). A multivariable logistic regression model was developed to predict granuloma in IPN.

Results

Can

SUBMITTER: Shipe ME 

PROVIDER: S-EPMC8162727 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature