Dataset Information

The sporothriolides. A new biosynthetic family of fungal secondary metabolites.

ABSTRACT: The biosynthetic gene cluster of the antifungal metabolite sporothriolide 1 was identified from three producing ascomycetes: Hypomontagnella monticulosa MUCL 54604, H. spongiphila CLL 205 and H. submonticulosa DAOMC 242471. A transformation protocol was established, and genes encoding a fatty acid synthase subunit and a citrate synthase were simultaneously knocked out which led to loss of sporothriolide and sporochartine production. In vitro reactions showed that the sporochartines are derived from non-enzymatic Diels-Alder cycloaddition of 1 and trienylfuranol A 7 during the fermentation and extraction process. Heterologous expression of the spo genes in Aspergillus oryzae then led to the production of intermediates and shunts and delineation of a new fungal biosynthetic pathway originating in fatty acid biosynthesis. Finally, a hydrolase was revealed by in vitro studies likely contributing towards self-resistance of the producer organism.

SUBMITTER: Tian DS

PROVIDER: S-EPMC8162735 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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The sporothriolides. A new biosynthetic family of fungal secondary metabolites.

Tian Dong-Song DS Kuhnert Eric E Ouazzani Jamal J Wibberg Daniel D Kalinowski Jörn J Cox Russell J RJ

Chemical science 20201021 46

The biosynthetic gene cluster of the antifungal metabolite sporothriolide 1 was identified from three producing ascomycetes: Hypomontagnella monticulosa MUCL 54604, H. spongiphila CLL 205 and H. submonticulosa DAOMC 242471. A transformation protocol was established, and genes encoding a fatty acid synthase subunit and a citrate synthase were simultaneously knocked out which led to loss of sporothriolide and sporochartine production. In vitro reactions showed th ...[more]

PMID: 34123230

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Dataset Information

The sporothriolides. A new biosynthetic family of fungal secondary metabolites.

Publications

The sporothriolides. A new biosynthetic family of fungal secondary metabolites.

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