2'-<i>O</i>-Trifluoromethylated RNA - a powerful modification for RNA chemistry and NMR spectroscopy.

ABSTRACT: New RNA modifications are needed to advance our toolbox for targeted manipulation of RNA. In particular, the development of high-performance reporter groups facilitating spectroscopic analysis of RNA structure and dynamics, and of RNA-ligand interactions has attracted considerable interest. To this end, fluorine labeling in conjunction with ¹⁹F-NMR spectroscopy has emerged as a powerful strategy. Appropriate probes for RNA previously focused on single fluorine atoms attached to the 5-position of pyrimidine nucleobases or at the ribose 2'-position. To increase NMR sensitivity, trifluoromethyl labeling approaches have been developed, with the ribose 2'-SCF₃ modification being the most prominent one. A major drawback of the 2'-SCF₃ group, however, is its stron

SUBMITTER: Himmelstoß M 

PROVIDER: S-EPMC8162808 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature