Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop-mediated isothermal nucleic acid amplification microfluidic device.

ABSTRACT:

Background

Rapid point-of-care (POC) detection of Streptococcus equi subsp. equi (S. equi) would theoretically reduce the spread of strangles by identifying index and carrier horses.

Hypothesis

That the eqbE isothermal amplification (LAMP) assay, and the same eqbE LAMP assay tested in a microfluidic device format, are comparable to a triplex real-time quantitative polymerase chain reaction (qPCR) assay that is commonly used in diagnostic labs.

Samples

Sixty-eight guttural pouch lavage (GPL) specimens from horses recovering from strangles.

Methods

Guttural pouch lavage specimens were tested for S. equi retrospectively using the benchtop eqbE LAMP, the eqbE LAMP microfluidic device, and compared to the triplex qPCR, that detects 2 S. equi-specific genes, eqbE and

SUBMITTER: Boyle AG 

PROVIDER: S-EPMC8163136 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature