Changes of the tRNA Modification Pattern during the Development of Dictyostelium discoideum.

ABSTRACT: Dictyostelium discoideum is a social amoeba, which on starvation develops from a single-cell state to a multicellular fruiting body. This developmental process is accompanied by massive changes in gene expression, which also affect non-coding RNAs. Here, we investigate how tRNAs as key regulators of the translation process are affected by this transition. To this end, we used LOTTE-seq to sequence the tRNA pool of D. discoideum at different developmental time points and analyzed both tRNA composition and tRNA modification patterns. We developed a workflow for the specific detection of modifications from reverse transcriptase signatures in chemically untreated RNA-seq data at single-nucleotide resolution. It avoids the comparison of treated and untreated RNA-seq data using rev

SUBMITTER: Hoffmann A 

PROVIDER: S-EPMC8163159 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature