A single amino acid Gly-tag enables metal-free protein purification.

ABSTRACT: Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C-C bond dissociation by resonance-assisted electron density polarization.

SUBMITTER: Purushottam L 

PROVIDER: S-EPMC8163197 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature