Melting temperature measurement and mesoscopic evaluation of single, double and triple DNA mismatches.

ABSTRACT: Unlike the canonical base pairs AT and GC, the molecular properties of mismatches such as hydrogen bonding and stacking interactions are strongly dependent on the identity of the neighbouring base pairs. As a result, due to the sheer number of possible combinations of mismatches and flanking base pairs, only a fraction of these have been studied in varying experiments or theoretical models. Here, we report on the melting temperature measurement and mesoscopic analysis of contiguous DNA mismatches in nearest-neighbours and next-nearest neighbour contexts. A total of 4032 different mismatch combinations, including single, double and triple mismatches were covered. These were compared with 64 sequences containing all combinations of canonical base pairs in the same location under the same con

SUBMITTER: Oliveira LM 

PROVIDER: S-EPMC8163305 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature