Unknown

Dataset Information

0

A synthetic glycan array containing Cryptococcus neoformans glucuronoxylomannan capsular polysaccharide fragments allows the mapping of protective epitopes.

ABSTRACT: A convergent synthetic strategy to Cryptococcus neoformans glucuronoxylomannan (GXM) capsular polysaccharide part structures was developed based on di-, tri-, tetra-, penta- and hexasaccharide thioglycoside building blocks. The approach permitted the synthesis of a library of spacer-containing serotype A and D related GXM oligosaccharide structures, ranging from di- to octadecasaccharides. Ten deprotected GXM compounds (mono- to decasaccharide) were printed onto microarray plates and screened with seventeen mouse monoclonal antibodies (mAbs) to GXM. For the first time a GXM oligosaccharide structure (a serotype A decasaccharide), capable of being recognized by neutralizing forms of these GXM-specific mAbs, has been identified, offering insight into the binding epitopes of a range of protective monoclonal antibodies and furthering our efforts to develop semi-synthetic conjugate vaccine candidates against C. neoformans.

SUBMITTER: Guazzelli L 

PROVIDER: S-EPMC8163368 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

A synthetic glycan array containing <i>Cryptococcus neoformans</i> glucuronoxylomannan capsular polysaccharide fragments allows the mapping of protective epitopes.

Guazzelli Lorenzo L   Crawford Conor J CJ   Ulc Rebecca R   Bowen Anthony A   McCabe Orla O   Jedlicka Anne J AJ   Wear Maggie P MP   Casadevall Arturo A   Oscarson Stefan S  

Chemical science 20200807 34

A convergent synthetic strategy to <i>Cryptococcus neoformans</i> glucuronoxylomannan (GXM) capsular polysaccharide part structures was developed based on di-, tri-, tetra-, penta- and hexasaccharide thioglycoside building blocks. The approach permitted the synthesis of a library of spacer-containing serotype A and D related GXM oligosaccharide structures, ranging from di- to octadecasaccharides. Ten deprotected GXM compounds (mono- to decasaccharide) were printed onto microarray plates and scre  ...[more]

Similar Datasets

Unknown EDTA inhibits biofilm formation, extracellular vesicular secretion, and shedding of the capsular polysaccharide glucuronoxylomannan by Cryptococcus neoformans.
| S-EPMC3485937 | biostudies-literature
Unknown Analysis of human monoclonal antibodies elicited by vaccination with a Cryptococcus neoformans glucuronoxylomannan capsular polysaccharide vaccine.
| S-EPMC173409 | biostudies-other
Unknown Self-aggregation of Cryptococcus neoformans capsular glucuronoxylomannan is dependent on divalent cations.
| S-EPMC1951138 | biostudies-literature
Unknown Direct inhibition of T-cell responses by the Cryptococcus capsular polysaccharide glucuronoxylomannan.
| S-EPMC1635532 | biostudies-literature
Unknown Capsular localization of the Cryptococcus neoformans polysaccharide component galactoxylomannan.
| S-EPMC2620754 | biostudies-literature
Unknown Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
| S-EPMC4906494 | biostudies-literature
Unknown Variable Region Identical IgA and IgE to Cryptococcus neoformans Capsular Polysaccharide Manifest Specificity Differences.
| S-EPMC4424345 | biostudies-literature
Unknown Automated glycan assembly of <i>Streptococcus pneumoniae</i> type 14 capsular polysaccharide fragments.
| S-EPMC9054924 | biostudies-literature
Unknown Molecular and idiotypic analysis of antibodies to Cryptococcus neoformans glucuronoxylomannan.
| S-EPMC303042 | biostudies-other
Unknown Binding of the wheat germ lectin to Cryptococcus neoformans suggests an association of chitinlike structures with yeast budding and capsular glucuronoxylomannan.
| S-EPMC2292635 | biostudies-literature