Examining histone modification crosstalk using immobilized libraries established from ligation-ready nucleosomes.

ABSTRACT: Chromatin signaling relies on a plethora of posttranslational modifications (PTM) of the histone proteins which package the long DNA molecules of our cells in reoccurring units of nucleosomes. Determining the biological function and molecular working mechanisms of different patterns of histone PTMs requires access to various chromatin substrates of defined modification status. Traditionally, these are achieved by individual reconstitution of single nucleosomes or arrays of nucleosomes in conjunction with modified histones produced by means of chemical biology. Here, we report an alternative strategy for establishing a library of differentially modified nucleosomes that bypasses the need for many individual syntheses, purification and assembly reactions by installing modified histone tails

SUBMITTER: Aparicio Pelaz D 

PROVIDER: S-EPMC8163371 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature