Construction of coacervate-in-coacervate multi-compartment protocells for spatial organization of enzymatic reactions.

ABSTRACT: Coacervate microdroplets, formed via liquid-liquid phase separation, have been extensively explored as a compartment model for the construction of artificial cells or organelles. In this study, coacervate-in-coacervate multi-compartment protocells were constructed using four polyelectrolytes, in which carboxymethyl-dextran and diethylaminoethyl-dextran were deposited on the surface of as-prepared polydiallyldimethyl ammonium/deoxyribonucleic acid coacervate microdroplets through layer-by-layer assembly. The resulting multi-compartment protocells were composed from two immiscible coacervate phases with distinct physical and chemical properties. Molecule transport experiments indicated that small molecules could diffuse between two coacervate phases and that macromolecular enzymes cou

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8163383 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature